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KMID : 1094720060110050466
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Biotechnology and Bioprocess Engineering
2006 Volume.11 No. 5 p.466 ~ p.469
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The performance of anion exchange expanded bed adsorption chromatography on the recovery of G6PDH from unclarified feedstock with high biomass concentration
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Chow Yen Mei
Tey Beng Ti
Mohd Nordin Ibrahim
Ariff Arbakariya
Ling Tau Chuan
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Abstract
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The bed stability of Streamline DEAE (p=1.2 g/mL) in a 20 mm (i.d.) glass expanded bed contactor, and its performance on the recovery of glucose 6-phosphate dehydrogenase (G6PDH) from unclarified yeast homogenate were investigated. A residence time distribution study showed that a stable expanded bed was achieved. The theoretical plate and Bodenstein numbers determined were 25 and 53, respectively. A recovery yield of 87% and purification factor of 4.1 were achieved in the operation using 5% (w/v) biomass concentration feedstock. The performance of the anion exchange EBAC was still considerable good at a biomass concentration as high as 15% (w/v).
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KEYWORD
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expanded bed adsorption, G6PDH, biomass, anion exchange, yeast
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